Measurement

Part:BBa_K3832009:Design

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-10-21)


trp-induce (detection circuit of tryptophan)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
    Illegal NotI site found at 2084
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1091
    Illegal SpeI site found at 1099
    Illegal SpeI site found at 2077
    Illegal AgeI site found at 915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 490
    Illegal SapI.rc site found at 1317


Design Notes

Source

Basic parts are from E.coli DH5a strain by PCR, and assemblied by useing GoldeGate assembly method.

References